Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against human Lewis a and Lewis b blood group antigens
Cellular Products
immunoglobulin; monoclonal antibody; against human Lewis a and Lewis b blood group antigens
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Blaszczyk M, et al. Lewis blood group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch. Biochem. Biophys. 233: 161-168, 1984. PubMed: 6465894
