| 產(chǎn)品名稱 |
15-79-2 |
| 商品貨號(hào) |
B163583 |
| Organism |
Mus musculus (pre-B cell); Mus musculus (myeloma), mouse (pre-B cell); mouse (myeloma) |
| Tissue |
liver |
| Cell Type |
hybridoma: pre-B lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
fetus |
| Applications |
The cells produce, but do not secrete mu chain. No light chain is produced. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Fetal liver cells (14 days gestation) were fused with P3X63Ag8.653 myeloma cells. No light chain is produced. |
| Genes Expressed |
immunoglobulin; mu heavy chain (not secreted) |
| Cellular Products |
immunoglobulin; mu heavy chain (not secreted) |
| Comments |
Fetal liver cells (14 days gestation) were fused with P3X63Ag8.653 myeloma cells. The cells produce, but do not secrete mu chain. No light chain is produced. The kappa light chain genes have not been functionally re-arranged. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 80%; fetal bovine serum, 20%. |
| Subculturing |
Medium Renewal: Every 1 to 2 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor |
JF Kearney |
| Deposited As |
mouse (pre-B cell); mouse (myeloma) |
| References |
Burrows P, et al. Evidence that murine pre-B cells synthesize mu heavy chains but no light chains. Nature 280: 838-841, 1979. PubMed: 112480
Perry RP, et al. Organization and expression of immunoglobulin genes in fetal liver hybridomas. Proc. Natl. Acad. Sci. USA 78: 247-251, 1981. PubMed: 6787586
Maki R, et al. Immunoglobulin gene rearrangement in immature B cells. Science 209: 1366-1369, 1980. PubMed: 6774416
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