| 產品名稱 | NCI-H1975 [H-1975, H1975] |
|---|---|
| 商品貨號 | B165300 |
| Organism | Homo sapiens, human |
| Tissue | lung |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | adenocarcinoma; non-small cell lung cancer |
| Gender | female |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The line was established in July 1988. The tissue donor was a non-smoker. |
| Clinical Data | female The tissue donor was a non-smoker. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing | Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: 1:3 to 1:6
Medium Renewal: two to three times weekly |
| Cryopreservation | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| STR Profile | Amelogenin: X CSF1PO: 12 D13S317: 10,13 D16S539: 9,12 D5S818: 11,12 D7S820: 8,11 THO1: 7 TPOX: 8,11 vWA: 18 |
| Name of Depositor | AF Gazdar, JD Minna |
| Deposited As | Homo sapiens |
| Year of Origin | 1988 |
| References | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. Sordella R, et al. Gefitinib-sensitizing EGFR mutations in lung cancer activate anti-apoptotic pathways. Science 305: 1163-1167, 2004. PubMed: 15284455 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
