| Comments |
A/J mice were immunized with a 50-kilodalton receptor protein (Rp-a) for the group B coxsackieviruses (CB) isolated in a virus-receptor complex from detergent-solubilized HeLa cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The RmcB cell line was established in 1985 by R. L. Crowell.
The antibody reacts with human CAR (the receptor for coxsackie B virus and the binding protein for adenovirus 2 and 3).
The antibody blocks binding and infection by coxsackie B Virus, but not adenovirus.
Mouse monoclonal antibody (RmcB) protects HeLa cells and Buffalo green monkey kidney cells from infection by all six serotypes of CB. RmcB did not protect HeLa cells from infection by poliovirus, echovirus 6, or coxsackievirus A18. |
| References |
Bergelson JM, et al. Isolation of a common receptor for coxsackie B viruses and adenoviruses 2 and 5. Science 275: 1320-1323, 1997. PubMed: 9036860
Mapoles JE, et al. Purification of a HeLa cell receptor protein for group B coxsackieviruses. J. Virol. 55: 560-566, 1985. PubMed: 2991580
Hsu KH, et al. A monoclonal antibody specific for the cellular receptor for the group B coxsackieviruses. J. Virol. 62: 1647-1652, 1988. PubMed: 2451756
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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