| 產品名稱 |
T1 (174 x CEM.T1) |
| 商品貨號 |
B165810 |
| Organism |
Homo sapiens, human |
| Tissue |
lymphoblast |
| Cell Type |
B lymphoblast,T lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
2 [Cells contain Epstein-Barr virus viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
T1 is the parental line for the T2 (174 x CEM.T2) line (ATCC CRL-1992), and together the lines are useful for studying antigen processing and T cell recognition of class I major histocompatibility antigens. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line is a cloned hybrid between the 721.174 (variant of the LCL 721 B lymphoblastic cell line) and CEMR.3 (8-azaguanine and ouabain resistant clone of the CEM T lymphoblastic cell line). |
| Antigen Expression |
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 + |
| Genes Expressed |
HLA A2, Aw30, B5, Bw4, Bw6, DR7; CD7 + |
| Comments |
The cells express large amounts of HLA DR7 and HLA A2.
It does not express DR3.
|
| Complete Growth Medium |
Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X
CSF1PO: 11, 12
D13S317: 11, 12
D16S539:10,12
D5S818: 12, 14
D7S820: 10, 11, 15
THO1: 6, 9.3
TPOX: 8, 12
vWA: 14, 17 |
| Name of Depositor |
P Cresswell |
| Deposited As |
Homo sapiens |
| References |
Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841
|
