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Escherichia coli (Migula) Castellani and Chalmers
Escherichia coli (Migula) Castellani and Chalmers
規格:
貨期:
編號:B171474
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Escherichia coli (Migula) Castellani and Chalmers
商品貨號 B171474
Classification Enterobacteriaceae, Escherichia
Strain Designations JMB9 (pRK248cIts)
Application
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Preceptrol® no
Genotype F- deltatrpEB9 supE44 lacY1 thr-1 leuB6 (pRK248cIts) thi-1 fhuA21 lambda-
Type Strain no
Comments
Restriction digests of pRK248cIts give the following sizes (kb): EcoRI--11.0; SalI--11.0; NcoI--10.5, 0.5; XbaI--uncut; SphI--11.0.
The plasmid confers tetracycline resistance.
Contains the plasmid pRK248cIts encoding a temperature-sensitive lambda repressor. Because this plasmid is compatible with ColE1 replicons, the strain can serve as a host for plasmids with expression to be thermally regulated from a lambda promoter.
Medium ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
Name of Depositor H Bernard
References

Bernard HU, et al. Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pL promoter. Gene 5: 59-76, 1979. PubMed: 372049

Thomas CM, et al. Regions of broad-host-range plasmid RK2 whihc are essential for replication and maintenance. J. Bacteriol. 141: 213-222, 1980. PubMed: 6243621

Kahn M, et al. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol. 68: 268-280, 1979. PubMed: 232215

Bernard HU, Helinski DR. Use of the lambda phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles. Methods Enzymol. 68: 482-492, 1979. PubMed: 232223

Hans-Ulrich Bernard, personal communication

Douglas P Cerretti, personal communication

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