狂野欧美激情性xxxx欧美I 成人性生交大片免费看中文网站I 日韩视频一区二区在线I 国产中的精品av小宝探花I 黄色国产在线I 久久精品视频在线观看I 在线天堂8√

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pSV2-hph
▍最近瀏覽歷史
▍聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
pSV2-hph
pSV2-hph
規格:
貨期:
編號:B171516
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pSV2-hph
商品貨號 B171516
Designations pSV2-hph
Depositors P Berg, M Dieckmann
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pSV2-hph (plasmid)
Promoters: Promoter SV40 early
Marker(s):ampR,hygR
Construct size (kb): 5.5
Features: marker(s): ampR, hygR
promoter: SV40 early
replicon: pMB1
terminator: SV40
enhancer: SV40 early
Applications
co-transfection marker aminocyclitol phoshotransferase hygromycin B phosphotransferase
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--3.87, 1.34, 0.69; PvuII/PstI--3.66, 1.84; PvuII--uncut.
An Escherichia coli/mammalian cell shuttle vector.
Consists of the SV40 promoter (SV nt 270-5171), a HindIII linker, the hph gene (1347 bp), SV40 splice site (SV nt 4710-4100), SV40 polyadenylation site (SV nt 4362-2067),
pML (inserted at the BamHI site with HindIII-EcoRI-amp upstream of the SV40 promoter).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gritz L, Davies J. Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae. Gene 25: 179-188, 1983. PubMed: 6319235

Montrose-Rafizadeh C, et al. High potency antagonists of the pancreatic glucagon-like peptide-1 receptor. J. Biol. Chem. 272: 21201-21206, 1997. PubMed: 9261127

Paul Berg, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板： | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |