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Size (kb): 6.1059999465942380

Vector: pZilch-NLS (phagemid)

Promoters: Promoter CMV

Construction: pCATCH-NLS

Marker(s):ampR

Construct size (kb): 6.105999946594238

Features: marker(s): ampR
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: BamHI...XhoI
MCS: BglII...XmaI
signal peptide coding region: SV40 NLS
terminator: SV40
coding sequence: mutant pheS gene
epitope tag: FLAG

encodes removable tag for protein isolation

expression vector

vector for assessing nuclear localization signals

vector permitting construction of fusion proteins

vector permitting positive selection for inserts

vector permitting production of single-stranded DNA

Restriction digests of the clone give the following sizes (kb): BamHI--6.1; EcoRI--6.1.

The vector was contructed by inserting a 1185 bp SmaI/BamHI fragment from pKSS encompassing the mutant pheS allele into the pCATCH-NLS and modified further to generate polylinker and to incorporate Kozak sequence and initiation ATG codon.

Temperature: 37.0°C