| 產品名稱 |
pGIKS-BioB |
| 商品貨號 |
B173253 |
| Designations |
pGIKS-BioB |
| GenBank Number |
J04423
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
M Follettie |
| Applications |
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). |
| Vector |
Construct size (kb): 4.1 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: XhoI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references: Vector end (5'): XhoI Vector end (3'): EcoRI |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: bioB
Gene name: biotin synthetase
Contains complete coding sequence?: Y
Type of DNA: genomic
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: EcoRI linker
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: J04423
Nucleotides 1-1041 of the insert correspond to
nucleotides 2012-3052 of J04423. Insert lengths(kb): 1.10 Gene product: biotin synthetase [bioB] Insert end (5'): XhoI linker Insert end (3'): EcoRI linker |
| Insert Size (kb) |
1.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert includes nt 2012 - 3052 of Genbank J04423. The insert contains the following restriction sites (approximate kb from the 5' end): RsaI--0.33; DdeI--0.58, 0.82; SspI--1.02. Restriction digests of the clone give the following sizes (kb): EcoRI--4.1; EcoRI/XhoI--3.0, 1.1; XhoI--4.1. |
| Classification |
Enterobacteriaceae, Escherichia |
