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pPB101
pPB101
規(guī)格:
貨期:
編號:B176508
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pPB101
商品貨號 B176508
Designations pPB101
Depositors HM Temin
Biosafety Level 2
Vector Information
Size (kb): 12.2799997329711900
Vector: pPB101 (plasmid)
Promoters: Promoter SNV LTR
Construction: pSW210, p60B Sal
Marker(s):ampR,tetS
Construct size (kb): 12.30000019073486
Features: marker(s): ampR
promoter: SNV LTR
replicon: pMB1, SNV
Applications
contains sequence
expression vector
shuttle vector
Comments
The insert contains an internal SalI site about 860 bp from the EcoRI end.
Full length molecular clone of spleen necrosis virus genome in pBR322.
Full-length molecular clone of spleen necrosis virus genome in pBR322.
A plasmid expression vector constructed from spleen necrosis virus, an avian retrovirus.
An EcoRI/BglII double digest yields a 2.0 kb fragment containing an LTR and initiator ATG for the gag protein.
This was constructed by cloning the 7.7 kb SalI fragment from p60B Sal (containing the SalI fragment of clone 60B) into the SalI site of pSW210 (pBR322 containing the AvrII/SalI fragment of pSNV-60B), thus generating an insert with 2 LTRs.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Watanabe S, Temin HM. Construction of a helper cell line for avian reticuloendotheliosis virus cloning vectors. Mol. Cell. Biol. 3: 2241-2249, 1983. PubMed: 6318091

Bandyopadhyay PK, Temin HM. Expression from an internal AUG codon of herpes simplex thymidine kinase gene inserted in a retrovirus vector. Mol. Cell. Biol. 4: 743-748, 1984. PubMed: 6325894

Chen IS, Temin HM. Substitution of 5' helper virus sequences into non-rel portion of reticuloendotheliosis virus strain T suppresses transformation of chicken spleen cells. Cell 31: 111-120, 1982. PubMed: 6297744

O'Rear JJ, et al. Infectious and noninfectious recombinant clones of the provirus of SNV differ in cellular DNA and are apparently the same in viral DNA. Cell 20: 423-430, 1980. PubMed: 6248244

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