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EN9F10

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編號(hào):B176737

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產(chǎn)品名稱(chēng)	EN9F10
商品貨號(hào)	B176737
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue	spleen
Cell Type	hybridoma: B lymphoblast
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Strain	BALB/c
Applications	The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate. The antibody can be used to determine the toxic potential of agricultural commodities and forensic samples involving most ergot alkaloids.
Storage Conditions	liquid nitrogen vapor phase
Derivation	The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate. The antibody detects ergopeptine and lysergic acid derivatives.
Genes Expressed	immunoglobulin; monoclonal antibody; against ergonovine (an ergot alkaloid)
Cellular Products	immunoglobulin; monoclonal antibody; against ergonovine (an ergot alkaloid)
Comments	The EN9F10 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with an ergonovine - human serum albumin conjugate. The antibody detects ergopeptine and lysergic acid derivatives. The antibody can be used to determine the toxic potential of agricultural commodities and forensic samples involving most ergot alkaloids.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 1 x 10⁵ and 1 x 10⁶ cells/mL. Do not allow the cell concentration to exceed 1 x 10⁶ cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Isotype	mouse IgG2b
Name of Depositor	RA Shelby
Deposited As	mouse (B cell); mouse (myeloma)
References	Shelby RA, Kelley VC. Detection of ergot alkaloids in tall fescue by competitive immunoassay with a monoclonal antibody. Food Agric. Immunol. 3: 169-177, 1991. Shelby RA, Kelley VC. Detection of ergot alkaloids from Clavicops species in agricultural products by competitive ELISA using a monoclonal antibody. J. Agric. Food Chem. 40: 1090-1092, 1992. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
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