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Size (kb): 5.0819997787475590

Vector: pSNATCH (phagemid)

Promoters: Promoter CMV

Construction: pSCTEV, pSuperCATCH-NLS

Marker(s):ampR

Construct size (kb): 5.081999778747559

Features: marker(s): ampR
other: VP16 activation domain
promoter: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: M13
replicon: SV40 ori
replicon: pMB1
replicon: polyoma
MCS: SfuI...XbaI
signal peptide coding region: SV40 NLS
terminator: SV40
epitope tag: FLAG

encodes removable tag for protein isolation

expression vector

vector for assessing nuclear localization signals

vector permitting construction of fusion proteins

vector permitting production of single-stranded DNA

Restriction digests of the clone give the following sizes (kb): EcoRI--5.1; HindIII--5.1; PstI--3.7, 1.4.

The vector was constructed as follows: a 120bp VP16 fragment from pSCTEV Gal4p/VP16 was ligated to adaptors and cloned into BamHI cut pSuperCATCH-NLS.

Temperature: 37.0°C

component of:ATCC 87677