| 產品名稱 |
Pochonia chlamydosporia var. chlamydosporia (Goddard) Zare et Gams |
| 商品貨號 |
B178147 |
| Strain Designations |
123 |
| Alternate State |
Metacordyceps chlamydosporia (Evans) Sung et al. |
| Application |
Biocontrol agent of plant parasitic nematodes. |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section |
| Type Strain |
no |
| Preceptrol® |
no |
| Genome Sequenced Strain |
Yes
|
| Comments |
Egg-parasitic nematophagous fungus. Genome sequencing strain (Donnelly Sequencing Centre, University of Toronto, Canada). |
| Morphology |
On PDA medium at 25°C after 7 days, mycelium white to pale yellow, very dense, raised, velvety. Produces a clear exudates after 7 days. Reverse mustard yellow to bright yellow center with pale yellow margin. Hyphae guttulate. Clamp connections not observed. |
| Medium |
ATCC® Medium 307: Cornmeal agar
ATCC® Medium 336: Potato dextrose agar (PDA)
ATCC® Medium 337: Potato, dextrose, yeast agar (PDY)
|
| Growth Conditions |
Temperature: 20°C to 25°C Atmosphere: Typical aerobic |
| Sequenced Data |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.
TAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTTCAACTCCCAAACCCCATGTGAACTTATACCATTTACAACCGTTGCTTCGGCGGGTTCTCGCCCCGGGCTTTACACCCCGGAACCAGGCGGCCCGCCGGGGGACCCAAACTCTAGATTTTTATTTTAGCATGTCTGAGTGGAATCATTACAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTCGAGCGTCATTTCAACCCTCAAGCCCCAGCGGTTTGGTGTTGGGGACCGGCGAGTACAGAGGCTTTGGGGACTTGTCCCCCTTCCCTCGGCGCCGCCCCCGAAATGAATTGGCGGTCTCGTCGCGGCCTCCTCTGCGTAGTAGCACAACCTCGCATCAGGAGCGCGGCGCGGCCACTGCCGTAAAACGCCCAACTTTTTTTAAGAGTTGACCTCGAATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGG
D1D2 region of the 28S ribosomal RNA gene:
TTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCCTAGGGCCCGAGTTGTAATTTGCAGAGGATGCTTTTGGCGAGGTGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTTGGATGCCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATACCGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTTGAAAGGGAAGCACTTATGACCAGACTTGGACCCGGTGAATCATCCAGCGTTCTCGCTGGTGCACTTTGCCGGGCTCAGGCCAGCATCAGTTTGCCTCGGGGGATAAAGGCTTTGGGAATGTGGCTCCCTCGGGAGTGTTATAGCCCATTGCACAATACCCTGGGGCGGACTGAGGTTCGCGCATCTGCAAGGATGCTGG
|
| Name of Depositor |
LV Lopez-Llorca |
| Special Collection |
ATCC |
| Chain of Custody |
ATCC <-- LV Lopez-Llorca |
| Isolation |
Eggs from phytopathogenic nematode Heterodera avenae, Sevilla, Spain. |
| Cross References |
Nucleotide (GenBank) :
KC171356
ITS including 5.8S rRNA gene
Nucleotide (GenBank) :
KC171327
D1/D2 region of 28S rRNA gene
|
| References |
Lopez-Llorca LV, et al. Expression of serine proteases in egg-parasitic nematophagous fungi during barley root colonization. Fung. Genet. Biol. 47: 342-351, 2010.
Macia-Vicente JG, Jansson HB, and Lopez-Llorca LV. Assessing fungal root colonization for plant improvement. Plant Signal. Behav. 4(5): 445-447, 2009.
Palma-Guerrero J, et al. Chitosan increases conidiation in fungal pathogens of invertebrates. Appl. Microbiol. Biotechnol. 87: 2237-2245, 2010.
|
