Features: marker(s): ampR replicon: pMB1, f1 terminator: none
Applications
vector for constructing cDNA libraries
vector permitting production of single-stranded DNA
Comments
Plasmid vector for construction of cDNA libraries.
The f1 intergenic region for packaging of ssDNA was inserted into an Okayama & Berg vector. SV40 sequences have been removed.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Kowalski J, et al. Vectors for the direct selection of cDNA clones corresponding to mammalian cell mRNA of low abundance. Gene 35: 45-54, 1985. PubMed: 2863196
