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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 3.3810000419616700

DESCRIPTION OF VECTOR:
Intact vector size: 3.381
Type of vector: phagemid
Cloning sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Polylinker sites: EcoRI SacI Asp718 KpnI SmaI BamHI
Construction: pUK21, pET3b, pEZZ18
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: T7, ->, 82-98
ribosome-binding site: Shine-Dalgarno, ->, 148-153
restriction site: NdeI, 159-164
leader peptide: synthetic ZZ sequence of protein A, ->, 162-641
MCS: EcoRI...BamHI, ->, 642-670
transcription terminator: T7, ->, 673-795
other: M13 intergenic region, ->
marker(s): kanR, ->
replicon: M13

Vector: pExSec1 (phagemid)

Promoters: Promoter for in vitro transcription T7

Construction: pUK21, pET3b, pEZZ18

Marker(s):kanR

Construct size (kb): 3.38100004196167

Features: leader peptide: synthetic ZZ sequence of protein A
marker(s): kanR
other: M13 intergenic region
promoter for in vitro transcription: T7
replicon: M13
MCS: EcoRI...BamHI
restriction site: NdeI
ribosome-binding site: Shine-Dalgarno
transcription terminator: T7

expression vector

vector permitting RNA synthesis in vitro

vector permitting construction of fusion proteins

vector permitting production of single-stranded DNA

vector with sequences for protein export

Restriction digests of the clone give the following sizes (kb): BamHI--3.4; BglI/EcoRI--3.1, 0.3; EcoRI--3.4; BglII/EcoRV--3.2, 0.2.

Expression and secretion vector for E. coli.

Constructed using M13 replication and kanR selection mechanisms from pUK21, T7 regulatory sequences from a 271 bp BglII-EcoRV fragment of pET3b and leader peptide sequence and multiple cloning sites from pEZZ18.

Temperature: 37.0°C