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in another host, produces protein cyclin-dependent kinase inhibitor 1A (p21, Cip1) melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)

Construct size (kb): 5.139999866485596

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSXV
Intact vector size: 4.200
Type of vector: plasmid
Vector end: XhoI
Vector end: BamHI
Cloning sites: XhoI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: CMV
replicon: pMB1
terminator: tat
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1A
Other gene symbols: CIP1; WAF1*
Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.2
Tissue: B-lymphocyte (EBV-transformed)
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: BglII
Insert size (kb): 1.64
Cross references: DNA Seq. Acc.: L25610

Insert lengths(kb): 1.639999985694885

Tissue: B lymphocyte (EBV transformed)

Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) [CIP1]

Target Gene: cyclin-dependent kinase inhibitor 1A (p21, Cip1)

Restriction digests of the clone give the following sizes (kb): BglI--1.6, 1.1, 0.6, 0.5 (doublet), 0.3 (doublet), 0.2; EcoRI--4.4, 0.9; KpnI--4.8, 0.25; PvuII--4.0, 1.2; XhoI--5.2;

XbaI/NdeI--2.7, 2.0, 0.45; EcoRI/XhoI--3.3, 1.05, 0.9.

Digestion with EcoRI+XhoI yields a 1 kb fragment that can be used as a probe.

Contains the complete coding sequence (nt 79-573 of the sequence record).

When transfected into human cells, expresses the protein from a CMV promoter.