| 產(chǎn)品名稱(chēng) |
Feline leukemia virus |
| 商品貨號(hào) |
B181857 |
| Classification |
Retrovirus, MLV-Related Viruses, Mammalian type C subgenera |
| Agent |
Feline leukemia virus |
| Strain |
CT600 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Testicular tissue of asymptomatic Asian leopard cat |
| Product Format |
frozen 1.0 mL per vial |
| Storage Conditions |
Vapor phase of liquid nitrogen |
| Comments |
Persistently infected cell culture Cells grow as monolayer Use 0.1% trypsin with 0.02% EDTA to subculture |
| Effect on Host |
No CPE; detect virus by RT assay or antibody |
| Recommended Host |
Cat testicular cells |
| Growth Conditions |
Temperature: 37°C
Recommendations For Infection: Virus is persistently infected in a continuous cell line. To passage the virus, trypsinize cell culture with 0.025% Trypsin-EDTA Solution (ATCC® 30-2101™) and seed cells using the growth medium.
Incubation: Until cells are confluent |
| Name of Depositor |
S Rasheed, MB Gardner |
| Source |
Testicular tissue of asymptomatic Asian leopard cat |
| References |
Rasheed S, Gardner MB. Isolation of feline leukemia virus from a leopard cat cell line and search for retrovirus in wild felidae. J. Natl. Cancer Inst. 67: 929-933, 1981. PubMed: 6268887
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