Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 0.7; PvuII--2.8, 1.1; NdeI/HindIII--3.2, 0.7. The insert contains the following restriction sites (nt from the 5' end): NdeI--60; EcoRV--671, 678. Includes a portion of the coding sequence (corresponding to part of the carboxy terminal region). The complete coding region extends from nt 35-1933 of the published sequence. Subcloned from pAS3-9. The 5' end of the insert (which is the 5' portion of the carboxy portion of the ORF) is adjacent to the EcoRI site of the MCS. |
