| Vector |
Construct size (kb): 8.5600004196167 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7, T3
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DRD2
Genomic copy number: unique
Gene name: dopamine receptor D2
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 q22.2-q22.3
Type of DNA: genomic
Insert 5' end: EcoRI
Insert 3' end: EcoRI
Insert size (kb): 5.6
Cross references: Insert lengths(kb): 5.599999904632568 Gene product: dopamine receptor D2 [DRD2] Alleles: A1, B1, B2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.0; BamHI--8.0, 0.6; HindIII--8.6; PstI--4.0, 1.5, 1.35, 0.8, 0.5, 0.3; HindIII/PstI--3.4, 1.5, 1.3, 0.8, 0.8, 0.5, 0.3. The insert contains a 4.5Kb TaqI fragment. The downstream portion of pDRD2E5.6 overlaps the Suarez clone phD2-254. |
| References |
Hauge XY, et al. Detection and characterization of additional DNA polymorphisms in the dopamine D2 receptor gene. Genomics 10: 527-530, 1991. PubMed: 1679742
Suarez BK, et al. Linkage Disequilibria at the D2 dopamine receptor (DRD2) in alcoholics and controls. Genomics 19: 12-20, 1994. PubMed: 7910578
Judith R Kidd, personal communication
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