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pCMV-drosE2F1
pCMV-drosE2F1
規(guī)格:
貨期:
編號(hào):B184096
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCMV-drosE2F1
商品貨號(hào) B184096
Designations pCMV-drosE2F1
GenBank Number

U10184

Species Drosophila melanogaster, fruit fly
Depositors JR Nevins
Applications
in another host, produces protein E2F transcription factor 1
Vector
Construct size (kb): 9.100000381469727
Insert
DNA: cDNA
Insert lengths(kb): 4.400000095367432
Tissue: imaginal discs
Gene product: E2F transcription factor 1 [E2f1]
Target Gene: E2F transcription factor 1
Insert Size (kb) 4.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 4.5 (doublet); EcoRV--7.1, 1.9; HindIII--6.6, 2.4.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.36; BglII--1.78; EcoRV--1.15, 3.00; HindIII--1.90; PstI--1.09, 1.51, 2.75, 2.95; PvuI--2.73, 3.08; PvuII--1.31, 2.35, 2.72; SalI--0.93, 1.40; XhoI--0.46, 1.44.
References

Ohtani K, Nevins JR. Functional properties of a Drosophila homolog of the E2F1 gene. Mol. Cell. Biol. 14: 1603-1612, 1994. PubMed: 8114698

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