| Comments |
The insert contains the following unique sites (in bp from the T7 promoter side [5']): HindIII--6; SmaI--269; FokI--318; AccI--851; SacI--925; DraI--1025; StuI--1451; XhoI--1513; EcoRI--1576. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.5, 0.3; HindIII--4.6; BamHI--4.2, 0.4; PstI--4.6; SacI--3.8, 0.8; SmaI--4.4, 0.3. Contains the complete coding sequence, 487 bp 3' untranslated sequence, and about 100 nt poly(A). Under moderately stringent conditions (0.5 M NaCl, 65C), the probe detects a genomic EcoRI fragment larger than 12 kb in humans, a 9 kb fragment in chickens, an 8 kb fragment in mouse and other less prominent bands. |
| References |
Ryder K, et al. A gene activated by growth factors is related to the oncogene v-jun. Proc. Natl. Acad. Sci. USA 85: 1487-1491, 1988. PubMed: 3422745
Lau LF, Nathans D. Expression of a set of growth-rleated immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc. Proc. Natl. Acad. Sci. USA 84: 1182-1186, 1987. PubMed: 3469660
Howard TA, et al. A linkage map of mouse chromosome 8: further definition of homologous linkage relationships between mouse chromosome 8 and human chromosomes 8, 16, and 19. Genomics 10: 207-213, 1991. PubMed: 1675193
Daniel Nathans, personal communication
|
