Restriction digests of the clone give the following sizes (kb): BamHI--7.6; ClaI--6.3, 1.3; BglI--6.5, 1.1. When this plasmid is introduced into a bacterial cell the activity of a test promoter can be measured using the 'small' luxAB cassette (2.1 kb) as a reporter gene. pGBM7 expresses constitutively the luxCDE genes, providing the cell with saturating concentrations of the aldehyde substrate necessary for the reaction of bacterial luciferases. pGBM7 was constructed by inserting PCR amplified luxC and luxDE, including their ribosome binding sites, into pGBM3 (ATCC 87499) between the SalI and EcoRI sites. |
