This holding is a cell line derived from an RIII mouse mammary tumor that produces MTV. Seed stock Hoechst stain positive--suggests contamination with mycoplasma.
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Mammary tumor (adenocarcinoma) from soft spontaneous tumor in 10-month-old RIII mouse
Product Format
frozen
Storage Conditions
Liquid nitrogen vapor
Comments
This holding is a cell line derived from an RIII mouse mammary tumor that produces MTV. Seed stock Hoechst stain positive--suggests contamination with mycoplasma.
Passage virus by passaging (splitting) cell line. Release of the virus into the supernate is facilitated by dispersing the cells and storing them at 4C for 7 days.
Effect on Host
tumorigenicity: no effect in tissue culture
Recommended Host
mouse
Growth Conditions
Incubation: 2-3 days
Name of Depositor
EY Lasfargues
Source
Mammary tumor (adenocarcinoma) from soft spontaneous tumor in 10-month-old RIII mouse
References
Lasfargues EY, et al. An established R3 mouse mammary tumor cell line; kinetics of mammary tumor virus (MTV) production. Proc. Soc. Exp. Biol. Med. 139: 242-247, 1972. PubMed: 4332331
