| 產品名稱 |
pMT70 |
| 商品貨號 |
B186683 |
| Designations |
pMT70 |
| Species |
Mus musculus, mouse |
| Depositors |
R Nusse |
| Vector |
Construct size (kb): 5.400000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 2.400000095367432 Gene product: wingless-type MMTV integration site 1, human homolog [Int1] |
| Insert Size (kb) |
2.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Insert contains internal BamHI (1) and ClaI (1) sites. Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 2.4; EcoRI--5.4; HindIII--5.4; BamHI--5.4; consistent with deposited DNA. A new nomenclature system has been proposed for Int-1 and related genes. It has been suggested that Int-1 become Wnt-1. Contains cDNA of murine int-1 that encodes the complete protein. RNA can be synthesized in vitro from the SP6 vector promoter. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
van Ooyen A, Nusse R. Structure and nucleotide sequence of the putative mammary oncogene int-1; proviral insertions leave the protein-encoding domain intact. Cell 39: 233-240, 1984. PubMed: 6091914
Rijsewijk FA, et al. Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro. Nucleic Acids Res. 14: 693-702, 1986. PubMed: 3003692
Nusse R, et al. A new nomenclature for int-1 and related genes: the Wnt gene family. Cell 64: 231, 1991. PubMed: 1846319
|
