BamHI digestion yields 2 vector fragments (5.4, 5.2 kb) and the l.45 kb insert. Restriction digests of the clone give the following sizes (kb): NotI--uncut; BssHII--12.0; SfiI--7.4, 4.7; PstI--6.6, 3.2, 1.6; SacI--12.0; BamHI--5.2 doublet; 1.5; HindIII--8.0, 3.8; BglII--12.0. Vector contains EBV oriP and EBNA to ensure replication as episomes in human or simian cells, even if the cell line is not EBNA positive. Expresses hprt gene and complements the HPRT-defect of UC lymphoblastoid cells. |
