Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Genotype
F- guaB22 rpsL125 fol-200
Type Strain
no
Comments
Grows slowly because of reduced initiation of translation from the deficiency of N-formylmethionyl-tRNA-F. If revertants are present, they grow more rapidly, producing large colonies.
Host for plasmids containing dihydrofolate reductase genes.
A useful host for plasmids containing dihyrofolate reductase genes.
Medium
ATCC® Medium 2957: LB Medium with 50μg/ml Thymidine and 100μg/ml Streptomycin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor
R Ferone
References
Singer S, et al. Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli. J. Bacteriol. 164: 470-472, 1985. PubMed: 3900045
