Restriction digests of the clone give the following sizes (kb): BamHI--7.8; EcoRI--5.0, 2.4; SacI/XbaI--3.8, 3.2, 0.42. High copy number shuttle expression vector. this vector has the TEF promoter for expression, the LEU2 marker gene and the 2 micron replicon. It is distributed in E. coli. |
