| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.5; PstI--3.75, 1.5, 0.95, 0.7; BglI--2.6, 2.3, 1.7; BglII--5.9, 0.9; PvuII--3.5, 2.7, 0.6. The insert contains the following restriction sites (bp from the 5' end): BglI--819; BglII--540, 1390; HincII--2275; NcoI--706; PstI--732, 1383, 2330; PvuII--526, 1107; SacI--324, 378; SmaI--317. Corresponds to nt 214-2620 of the published sequence and contains the complete coding region except for the first four amino acids. Detects a single band of approximately 3.2 kb in Northern blots of RNA from human skin fibroblasts. |
