Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Storage Conditions
-70°C or colder
Comments
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
CPE, cells enlarge as inclusions form, which push cellular material outward, leaving a swollen, round middle. Inclusions best visualized by FA. Death of chicken embryo.
Recommended Host
McCoy (ATCC® CRL-1696™)
Growth Conditions
Recommendations for Infection: For best results in tissue culture, cells should be 24 to 48 hours old and 80% - 100% confluent. The cells will not continue to grow after adding the cycloheximide.
Incubation: In TC, 2 to 3 days at 37°C, a 5% CO2 in air atmosphere is recommended. In CE, 7 to 10 days at 35°C.
Name of Depositor
J Schachter
Source
Lymph node from human with LGV
References
Schachter J, et al. Lymphogranuloma venereum. I. Comparison of the Frei test, complement fixation test, and isolation of the agent. J. Infect. Dis. 120: 372-375, 1969. PubMed: 5822618
Schachter J, Meyer KF. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99: 636-638, 1969. PubMed: 4905529
