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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 9.0000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 9.000
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Construction: pNKY51, Tn903
Host range: Candida robustaSaccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, kanR, URA3
replicon: pMB1

Vector: pSE1076 (plasmid)

Construction: pNKY51, Tn903

Marker(s):URA3,ampR,kanR

Construct size (kb): 9.0

Features: marker(s): ampR, kanR, URA3
replicon: pMB1

vector for other uses

Restriction digests of the clone give the following sizes (kb): BglII/BamHI--5.2, 3.8; BamHI--9.0.

Restriction digests of the clone give the following sizes (kb): BglII--9.0; BamHI--9.0; BamHI/BglII--5.2, 3.8; SmaI--9.0.

Used by inserting the excised cassette into a cloned target gene, isolating a restriction fragment containing the cassette with flanking target gene sequences, transforming Ura3- strains, and selecting for stable integrants.

A plasmid containing an excisable URA3 cartridge for gene disruption that permits repeated use of URA3 selection in constructing multiply disrupted yeast strains.

A derivative of pNKY51 with kanR from Tn903 inserted at the SmaI site of pNKY51.

The order of the major features in this plasmid is: BglII (nt 1) - hisG - nt 1150 - URA3 - nt 2350 - kanR - nt 3850 - hisG - BamHI (nt 5000) - pMB1 ori - ampR.

Temperature: 37.0°C