Nucleotide (GenBank) :
VB0096
pICEM19H plus phasmid cloning vector.
Biosafety Level
1
Vector Information
Size (kb): 3.9730000495910650
Vector: pICEM19H+ (phagemid)
Construction: pIC7, pEMBL8+
Marker(s):ampR
Construct size (kb): 3.973000049591065
Features: insert detection: lacZ' marker(s): ampR replicon: pMB1, f1
Applications
vector permitting production of single-stranded DNA
Comments
Chimeric phage/plasmid for cloning into lacZ' polylinker for production of ssDNA.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Marsh JL, et al. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32: 481-485, 1984. PubMed: 6099327
