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Construct size (kb): 17.5

DNA: cDNA

Insert lengths(kb): 4.380000114440918

Tissue: liver cell line HEPG2

Gene product: paired basic amino acid cleaving enzyme (furin, membrane associated receptor protein)( paired basic amino acid cleaving enzyme, furin, paired basic amino acid cleaving enzyme) [FUR]

Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.0; BglII--0.6; EcoRV--3.2; KpnI--0.8, 4.15; NdeI--1.7; NcoI--0.4, 1.7, 1.9, 2.65; PstI--0.7, 2.6, 3.8; SmaI--0.3, 3.5; SphI--1.1; XhoI--1.8.

Insert encodes 388 bp of 5' untranslated sequence, a 794 aa coding sequence (nt 408 - 2789 of the GenBank record) and 1597 bp of 3' untranslated sequence.

Recommendation for verification: BamHI+SalI, KpnI, PstI, SmaI.