| Vector |
Construct size (kb): 4.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: HSPA1A
Other gene symbols: HSPA1
Gene name: heat shock 70kD protein 1
Contains complete coding sequence?: U
Chromosome: 6; Localization: 6 p21.3
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.7
Cross references: DNA Seq. Acc.: M59828; EST: EST01640
Nucleotides 1-182 of the insert correspond to
nucleotides 499-680 of M59828 with 100% identity.
DNA Seq. Acc.: M78053; EST: EST01640; Primer: M13 Forward;
THC id: THC83201 Insert lengths(kb): 1.700000047683716 Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205) Gene product: heat shock 70kD protein 1( heat shock 70kD protein 1A, heat shock-induced protein, heat shock protein HSP70-1, major histocompatibility complex,class III, HSP70-1) [HSPA1] Alleles: A1, A1, A2, A2 |
