Transient transfection, stable transfection, gene expression, protein production, genetic manipulation. Suitable for transfection of Bone Marrow MSCs (PCS-500-012), Dermal Microvascular Endothelial cells (PCS-110-010), THP-1 cells (TIB-202), Raw 264.7 cells (TIB-71), and SH-SY5Y cells (CRL-2266). A complete list of cell line-specific protocols can be found here.
Comments
This reagent affords high levels of gene expression in a variety of cell types and is suitable for both transient and stable transfection. GeneXPlus works effectively in large scale reactions, producing high protein yields. Since the reagent is composed of animal-origin free components and is serum compatible, there is no need for any culture medium change after transfection.
GeneXPlus Transfection Reagent has been optimized for use with HEK293T/17 SF suspension cells (ATCCACS-4500) and HEKPlus SFM (ATCCACS-4002) to reproducibly express a wide range of proteins at high levels.
Intended Use
This product is intended for research use only. It is not intended for any animal or human therapeutic or diagnostic use.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
GeneXPlus Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells.
Quality Control Specifications
Physical characterization of reagent components is performed using mass spectrophotometry and nuclear magnetic resonance (NMR) for identification and determination of purity. GeneXPlus is tested for performance using a luciferase reporter assay in HEK293 cells.
