| 產品名稱 |
pJJ283 [pJJ252] |
| 商品貨號 |
B189665 |
| Designations |
pJJ283 [pJJ252] |
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
L Prakash |
| Vector |
Construct size (kb): 4.688000202178955 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: XbaI
Vector end: XbaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: LEU2
Gene name: 3-isopropylmalate dehydrogenase
Contains complete coding sequence?: U
Chromosome: III; Localization: III L, 5 cM from CEN
Type of DNA: genomic
Insert 5' end: SalI
Insert 3' end: HpaI
Insert size (kb): 1.9
Cross references: Insert lengths(kb): 1.899999976158142 Gene product: 3-isopropylmalate dehydrogenase(beta-isopropylmalate dehydrogenase) [LEU2] |
| Insert Size (kb) |
1.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--2.7, 1.9; PvuII--2.3 (doublet); EcoRI--3.65, 0.95. A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions. Constructed by filling in the ends of both the vector and the insert fragment. LEU2 is in an orientation opposite to lacZ'. The insert was subcloned from YEp13. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072
Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
Louise Prakash, personal communication
|
