| Vector |
Construct size (kb): 5.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: NotI
Vector end: NotI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->, 3-459
primer site: M13-20, ->
promoter for in vitro transcription: T7, ->
insert detection: lacZ', <-, 816-938
MCS: SacI...KpnI, ->, 657-759
promoter for in vitro transcription: T3, <-
primer site: reverse, <-
replicon: pMB1, 1032-1972
marker(s): ampR, <-, 1975-2832
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SP2
Gene name: Sp2 transcription factor
Contains complete coding sequence?: Y
Tissue: Molt13 cell line
Type of DNA: cDNA
Insert end: NotI
Insert end: NotI linker
Insert size (kb): 2.7
Cross references: DNA Seq. Acc.: M97190
Nucleotides 1-2063 of the insert correspond to
nucleotides 1-2063 of M97190. Insert lengths(kb): 2.700000047683716 Tissue: Molt13 cell line Gene product: Sp2 transcription factor(Trans-acting transcription factor 2) [SP2] Target Gene: Sp2 transcription factor |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--2.75, 1.25, 1.05, 1.0; NotI--3.0 (doublet); PstI--4.8, 1.15; PvuII--2.6, 1.8, 0.76, 0.66 +smaller fragments; SphI--6.0. Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.29; PstI--0.07, 1.14; PvuII--0.34, 0.90, 1.05, 1.14, 1.22, 1.30, 1.39, 1.46; SacI--1.72, 2.06; SmaI--1.50; SphI--1.73. |
