| 產品名稱 |
pCMV-SREBP1c-436 |
| 商品貨號 |
B190060 |
| Designations |
pCMV-SREBP1c-436 |
| GenBank Number |
S66167S1
|
| Species |
Homo sapiens, human |
| Depositors |
JD Horton, H Shimano, JL Goldstein |
| Applications |
contains sequence sterol regulatory element binding transcription factor 1 SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1 |
| Vector |
Construct size (kb): 6.300000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV7
Intact vector size: 4.900
Type of vector: phagemid
Vector end: SmaI
Vector end: SmaI
Cloning sites: PstI, SalI, XbaI, BamHI, SmaI
Polylinker sites: PstI, SalI, XbaI, BamHI, SmaI
Features (with orientation and position when available):
enhancer: N701
MCS: PstI...SmaI
coding sequence: hGH
enhancer: SV40
marker(s): ampR, <-
replicon: f1, <-
promoter: CMV
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: SREBF1 sterol regulatory element binding transcription
factor 1
Contains complete coding sequence?: N
Chromosome: 17; Localization: 17 p11.2-p11.2
Type of DNA: cDNA
Insert end: SalI-blunted
Insert end: SalI-blunted
Insert size (kb): 1.4
Cross references: DNA Seq. Acc.: S66167S1 Insert lengths(kb): 1.399999976158142 Gene product: sterol regulatory element binding transcription factor 1( SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1) Target Gene: sterol regulatory element binding transcription factor 1 |
| Insert Size (kb) |
1.400 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.3; SalI--4.9, 1.4; PstI--5.8, 0.35, 0.33; PstI/XbaI--4.9, 0.9, 0.5; XbaI--6.3. Insert encodes amino acids 1-436 of human SREBP-1c cDNA and 5' UTR. Insert sequence also corresponds to Genbank U00968. |
| References |
Shimano H, et al. Isoform 1c of sterol regulatory element binding protein is less active than isoform 1a in livers of transgenic mice and in cultured cells. J. Clin. Invest. 99: 846-854, 1997. PubMed: 9062341
|
