Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
After in vitro packaging and transfection, cosmid libraries may be established by antibiotic selection at 32C. Libraries may be subsequently packaged directly in vivo by shifting to 42C.
A lambda lysogen of DK1 (ATCC 35691)
After in vitro packaging and transfection, cosmid libraries may be established by antibiotic selection at 32C. Libraries may be subsequently packaged directly in vivo by shifting to 42C.
This recA- host strain is a useful in vivo packaging host for cosmids that utilize lambda cohesive ends.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
Name of Depositor
DM Kurnit
References
Kurnit DM. Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging. Gene 82: 313-315, 1989. PubMed: 2684784
