| 產品名稱 |
Vaccinia virus ts mutant |
| 商品貨號 |
B190375 |
| Classification |
Poxviridae, Orthopoxvirus |
| Agent |
Vaccinia virus ts mutant |
| Strain |
IHD-W Dts42 |
| Applications |
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location G2, a viral positive transcription elongation factor. |
| Biosafety Level |
2 BSL 2Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This conditional lethal mutant, originally designated plaque isolate no. 6626, was isolated from the IHD-W strain of Vaccinia by S. Dales. The IHD-W strain was derived from an IHD-J strain of Vaccinia. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
This conditional lethal mutant, originally designated plaque isolate no. 6626, was isolated from the IHD-W strain of Vaccinia by S. Dales by mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine (Dales, et al, 1978). The non-permissive incubation temperature is 39.5 C. This mutant was assigned to Dales EM categories D, inducing foci of viroplasma and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location G2, a viral positive transcription elongation factor. In the absence of G2 protein, transcripts are shorter than in the presence of G2 protein.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox and cowpox viruses |
| Effect on Host |
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing CPE: enlargement, plaques with rounding and eventual sloughing |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
|
| Recommended Host |
Production Host: BSC-40 (ATCC CRL-2761) Recommended Host: BSC-40 (ATCC CRL-2761) Alternate Host(s): MRC-5, BS-C-1, HeLa, LLC-MK2 |
| Growth Conditions |
1-3 days at 31 C with 5% CO2 |
| Name of Depositor |
S. Dales, R. Condit |
| References |
Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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