Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The depositor recommends picking a single colony from a plate containing tetracycline to grow for stock.
A recombination-proficient host for use with lambdaZd vectors (ATCC 37563-37565) and for releasing plasmid inserts from lambdaZd libraries and clones. Incoming lambdaZd phage are maintained as large plasmids at 32C.
a host containing a temperature sensitive lambda repressor gene (cI857) on a ColE1-compatible plasmid
Medium
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
Name of Depositor
AJ Murphy
References
Bernard HU, Helinski DR. Use of the lambda phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles. Methods Enzymol. 68: 482-492, 1979. PubMed: 232223
Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791
