Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.1, 1.15; HindIII--5.2; SalI--5.2; PstI--5.2; EcoRI--5.2. Expression vector permitting visual detection of recombinants when plated on skim milk indicator plates by the loss or reduction of a halo around a colony. Contains the signal sequence for the neutral protease, so that recombinants in-frame with the gene may be exported. Constructed by an in-frame replacement of part of the pro-peptide coding region with part of the MCS from pUC19. The order of the major features in this plasmid is: nprE (signal peptide coding region - MCS - pro peptide coding region - mature peptide coding region) - ori - kanR. |
