For in situ hybridization with metaphase chromosomes, a larger insert is recommended.
Vector
Construct size (kb): 3.299999952316284
Insert
DNA: genomic
Insert lengths(kb): 0.3799999952316284
Gene product: DNA Segment, repetitive [D22Z3]
Insert Size (kb)
0.380
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): SphI--3.3; HindIII--3.3; PstI--3.3; EcoRI--3.3; EcoRI/PstI--2.9, 0.38.
This insert does not hybridize to mouse DNA or to DNA from Callithrix jacchus or Cercopithecus aethiops. Multiple EcoRI bands were detected in Southern blots from Pan troglodytes and Pongo pygmaeus.
This insert contains a tandemly organized 48 bp sequence, repeated 8 times. The consensus sequence is cagaacactgcta(c/t)gagggtctgaatgtttgtccctcacataggattc.
This insert is suitable for hybridization with filters. For in situ hybridization with metaphase chromosomes, a larger insert is recommended.
References
Metzdorf R, et al. A novel centromeric repetitive DNA from human chromosome 22. Chromosoma 97: 154-158, 1988. PubMed: 3229178
