| 產品名稱 |
pRat alpha 2 |
| 商品貨號 |
B191288 |
| Designations |
pRat alpha 2 |
| GenBank Number |
M14512
|
| Species |
Rattus norvegicus, rat |
| Depositors |
JB Lingrel |
| Applications |
isoform-specific probe ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Vector |
Construct size (kb): 3.16700005531311 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: SmaI
Vector end: SmaI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: rat
Gene symbol: ATP1A2
Gene name: ATPase, Na+/K+ transporting, alpha 2 polypeptide
Contains complete coding sequence?: N
Strain: CD
Tissue: brain
Type of DNA: cDNA
Insert 5' end: ScaI
Insert 3' end: NheI Modification: blunted
Insert size (kb): 0.381
Cross references: DNA Seq. Acc.: M14512
Nucleotides 1-381 of the insert correspond to
nucleotides 121-502 of M14512. Insert lengths(kb): 0.3810000121593475 Tissue: brain Gene product: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide [ATP1A2] Target Gene: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Insert Size (kb) |
0.381 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
This is a 5' specific probe for the alpha2 isolform. It is not full length. Nucleotides 1-381 of the insert correspond to nucleotides 121-502 of Genbank M14512. The insert contains the following restricti sites (approximate kb from the 5' end): SacI--0.06, 0.17; NcoI--0.09, 0.33. Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.7, 0.4; BglI--2.0, 1.2; PvuII--2.4, 0.7. |
| References |
Young RM, Lingrel JB. Tissue distribution of mRNAs encoding the alpha isoforms and beta subunit of Rat Na+, K+-ATPase. Biochem. Biophys. Res. Commun. 145: 52-58, 1987. PubMed: 3036133
Orlowski J, Lingrel JB. Tissue-specific and developmental regulation of Rat Na,K-ATPase catalytic alpha isoform and beta subunit mRNAs. J. Biol. Chem. 263: 10436-10442, 1988. PubMed: 2839491
Shull GE, et al. Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha subunit from Rat brain. Biochemistry 25: 8125-8132, 1986. PubMed: 3028470
|
