| 產(chǎn)品名稱 |
pB4 |
| 商品貨號 |
B191462 |
| Designations |
pB4 |
| GenBank Number |
M13994
|
| Species |
Homo sapiens, human |
| Depositors |
CM Croce |
| Vector |
Construct size (kb): 3.609999895095825 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: BCL2
Genomic copy number: unique
Gene name: B cell CLL/lymphoma 2
Contains complete coding sequence?: U
Chromosome: 18; Localization: 18 q21.3
Tissue: cell line 380
Type of DNA: cDNA
Insert end: EcoRI Modification: EcoRI linkers
Insert end: EcoRI Modification: EcoRI linkers
Insert size (kb): 0.91
Cross references: DNA Seq. Acc.: M13994 Insert lengths(kb): 0.9100000262260437 Tissue: cell line 380 Gene product: B cell CLL/lymphoma 2 [BCL2] |
| Insert Size (kb) |
0.910 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 1.0; BglI--1.9, 1.2, 0.54; PstI--3.0, 0.62; BamHI--3.3, 0.38; XbaI--3.7. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.4; PstI--0.3; PvuII--0.45; SacI--0.5; BamHI--0.65. Contains approximately nt 1410 to 2340 of the published sequence. |
| References |
Reed JC, et al. BCL2-mediated tumorigenicity of a human T-lymphoid cell line: synergy with MYC and inhibition by BCL2 antisense. Proc. Natl. Acad. Sci. USA 87: 3660-3664, 1990. PubMed: 1692620
Tsujimoto Y, Croce CM. Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma. Proc. Natl. Acad. Sci. USA 83: 5214-5218, 1986. PubMed: 3523487
|
