| Insert |
DNA: cDNA Insert lengths(kb): 2.799999952316284 Tissue: melanoma B16 cell line Gene product: brown(b-locus protein, catalase 2, tyrosinase-related protein) [b] Alleles: A2, A2, A1, A1, A2, A1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI/PvuII--1.5, 1.08, 0.92, 0.87, 0.72, 0.29; XbaI--4.4, 1.1; BamHI--5.8; KpnI--5.8; HindIII--2.7, 1.6, 1.1. The insert contains the following restriction sites (nt from the 5' end): XbaI--574, 1682; HindIII--793, 2361; KpnI--853; PstI--894; BamHI--974. The vector lacks the SV40 promoter. Although the gene product is recognized by anti-b-locus protein antibodies, it does not have tyrosinase activity. It has been called tyrosinase-related protein or b-locus protein. This is a full length cDNA clone. Coding sequences extend from nucleotides 116 through 1726. Enzyme(s) not detecting human polymorphism: StuI, BglII, HpaI, HaeII, PvuII, BamHI, PstI, HindIII, EcoRI, TaqI, AvaII, MspI (10 unrelated individuals). |
| References |
Cohen T, et al. Nucleotide sequence of the cDNA encoding human tyrosinase-related protein. Nucleic Acids Res. 18: 2807-2808, 1990. PubMed: 2111010
Shibahara S, et al. Cloning and expression of cDNA encoding mouse tyrosinase. Nucleic Acids Res. 14: 2413-2427, 1986. PubMed: 3008090
Colman MA, et al. A two allele XbaI RFLP at the catalase 2 locus. Nucleic Acids Res. 19: 960, 1991. PubMed: 1673236
Shibahara S, et al. Structural organization of the pigment cell-specific gene located at the brown locus in mouse. Its promoter activity and alternatively spliced transcript. J. Biol. Chem. 266: 15895-15901, 1991. PubMed: 1908461
Shigeki Shibahara, personal communication
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