Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI/HindIII--2.7, 1.5; HindIII--2.7, 1.5; PstI--3.5, 0.68; XbaI--4.2. Linkage has been demonstrated between X-linked recessive hydrocephalus (HSAS) and DXS52 (theta = 0.03, LOD score = 6.52). Linkage has also been demonstrated between HSAS and F8 (theta = 0.00, LOD score = 4.32). This is a hybrid probe containing a fragment of the factor VIII gene that detects the BclI RFLP and a fragment close to the St14 minisatellite that detects the VNTR in several restriction digests including TaqI, BclI, and MspI. This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels. It also gives a clear pattern for DXS52 on TaqI blots. This probe does not detect the 2-allele RFLPs of St14-1 (ATCC 61372) (TaqI, BclI). |
