| Vector Information |
Size (kb): 42.40 DESCRIPTION OF VECTOR:
Intact vector size: 42.400
Type of vector: phage
Cloning sites: XhoI EcoRI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Cloning capacity: 0 - 8.4 kb
Construction: lambdaYES-R, pADH
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: ADH, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: right arm (15.2 kb, with cI857) Vector: lambdaADH (phage, lambda - replacement) Promoters: Promoter for expression ADH Construction: lambdaYES-R, pADH Marker(s):URA3,ampR Construct size (kb): 42.40 Features: marker(s): URA3
marker(s): ampR
other: left arm (19.6 kb)
other: lox
other: right arm (15.2 kb, with cI857)
promoter for expression: ADH
promoter for expression: lac
replicon: ARS1
replicon: pMB1
MCS: XhoI EcoRI
transcription terminator: HIS3
centromere: CEN4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--43.0; SalI--21.0, 13.5, 13.0; XhoI--25.0, 21.0. Transcription initiated from the ADH promoter in yeast hosts can be terminated at the HIS3 terminators. Vector for the construction of cDNA libraries permitting regulated expression in both yeast and E. coli and simple conversion to plasmid via the cre-lox system. The plasmid derivative (pADH, 7.75 kb) is a YC-type shuttle expression vector. The lac promoter contains a ribosome binding sequence optimally spaced from an AUG start codon and can support translational fusions. |
