Vector maintained either as a self-replicating plasmid or as an integrated prophage, depending on the presence of a tRNA nonsense-codon suppressor in the host strain.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References
Ow DW, Ausubel FM. Conditionally replicating plasmid vectors that can integrate into the Klebsiella pneumoniae chromosome via bacteriophage P4 site-specific recombination. J. Bacteriol. 155: 704-713, 1983. PubMed: 6307977
