Restriction digests of the clone give the following sizes (kb): HindIII--4.6kb; BamHI--3.5, 1.2; EcoRI/XhoI--3.0, 1.7. The insert contains the following restriction sites (approximate from the 5' end): HindIII--0.45; EcoRV--1.03; BamHI--1.16; BglI--1.07, 0.06. Recommendation for verification: EcoRI/XhoI--2.94, 1.74; BamHI--3.51, 1.16; HindIII--4.67. Murine stromelysin-2 was isolated from a murine wound cDNA library screened with a 212-bp fragment of murine stromelysin-2 cDNA. |
