| Vector |
Name of construct: pTK-HSV-SCAP-T7
Excise insert: ClaI+NdeI
Promoter giving sense strand: TK
Size of construct (kb): 10.0
Markers: ampR, G418R
Name of vector: pTK-HSV/T7
Intact vector size: 6.0
Type of vector: phagemid
Vector end: ClaI
Vector end: NdeI
Cloning sites: ClaI NdeI
Cloning capacity: pcDNA3, TK, HSV
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter for expression: TK, ->
restriction site: NotI
promoter for in vitro transcription: T7, ->
stop codons: TAG, ->
restriction site: SpeI NheI
initiation codon: ATG, ->
epitope tag: HSV, ->
restriction site: ClaI
restriction site: NdeI
epitope tag: T7, ->
stop codons: TGA, ->
promoter for in vitro transcription: SP6, <-
terminator: bGH polyadenylation, ->
replicon: f1, ->
replicon: SV40, ->
promoter: SV40, ->
marker(s): G418R, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1
marker(s): ampR, <- |
| References |
Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195
Hua X, et al. Regulated cleavage of sterol regulatory element binding proteins requires sequences on both sides of the endoplasmid reticulum membrane. J. Biol. Chem. 271: 10379-10384, 1996. PubMed: 8626610
Joseph L Goldstein, personal communication
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